RNA sequencing: End Repair ingredients (Truseq)

[4N6Girl]

RNA sequencing: End Repair ingredients (Truseq)

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Post by 4N6Girl » Wed Jun 25, 2014 3:21 pm

Is anyone familiar with Illimuna's Truseq RNA sequencing library prep kit? I am always running out of End Repair, and I was wondering if it would just be easier to make the End Repair in-house. Does anyone know where I could find the ingredients? I can experiment with the ratios, but I have no idea what's in it (except DTT).

Thanks!