Extracellular domain or intracellular domain?
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Extracellular domain or intracellular domain?
I spent quite a while thinking how to determine which parts of a protein face outward from the plasma membrane and which parts face inwards?
How can I go about determining the intracellular domain and extracellular domain of a new protein?
Can anyone please help? Many thanks in advance!
How can I go about determining the intracellular domain and extracellular domain of a new protein?
Can anyone please help? Many thanks in advance!
1) you could use some bioinformatic approach
2) you could try some partial hydrolysis of your protein inserted into the membrane and by analysis of released domain/peptides, you should be able to say, which part is intra/extracellular
3) maybe (really not sure, whether this would work!), you could somehow color your protein and see it under microscope (but I don't think you would be able to distinguish extra/intra
)
4) if you know, whether it binds something, you could try to design some suicide ligand, which would bind covalently after bonding and by analysis of which peptide it contains, you should be able to see, which part is extra/intra
of course for all these approaches you need to work with some system, where you have inserted all proteins in the same way in intact bodies
2) you could try some partial hydrolysis of your protein inserted into the membrane and by analysis of released domain/peptides, you should be able to say, which part is intra/extracellular
3) maybe (really not sure, whether this would work!), you could somehow color your protein and see it under microscope (but I don't think you would be able to distinguish extra/intra
)4) if you know, whether it binds something, you could try to design some suicide ligand, which would bind covalently after bonding and by analysis of which peptide it contains, you should be able to see, which part is extra/intra
of course for all these approaches you need to work with some system, where you have inserted all proteins in the same way in intact bodies
http://www.biolib.cz/en/main/
Cis or trans? That's what matters.
Cis or trans? That's what matters.
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