## Dilution

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

carbonp
Garter
Posts: 7
Joined: Tue Oct 13, 2009 11:35 pm

### Dilution

Take 5 small glass test tubes (1-5) and put 1200 μl of distilled water in each.
Now add 300 μl of 10 μM atropine to Tube 1, mix well and transfer 300 μl to Tube 2.
Mix well and transfer 300 μl to Tube 3.
Repeat up to Tube 5.
Calculate the atropine concentration in each tube.

my question is
For first test tube,
C1 = 10 micromolar
V1 = 300 microliter
C2 = we are finding
V2 = 1200 or 1500 microliter?????????????????

mith
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:
you started with 1200, and you added 300ul fluid. for the purpose of the calculation you have 1500, however by distributing the mixed contents, your final volume in the tube is 1200.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

### Who is online

Users browsing this forum: SueCiz and 6 guests