northern blot analysis
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northern blot analysis
hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help...
i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean?
thanks in advance

i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean?
thanks in advance

- MrMistery
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maybe unprocessed and processed RNA?
I have never used northern blots (honestly I didn't know anyone still used them) so I'm just taking a guess here
I have never used northern blots (honestly I didn't know anyone still used them) so I'm just taking a guess here
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
Re: northern blot analysis
proteinz wrote:hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help...
i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean?
thanks in advance
Is it possible you are picking up a mRNA from a similar gene that produces a larger transcript?
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