Haematocrit and Packed Cell Volumes

micco13 · Post by **micco13** » Mon Oct 06, 2008 8:53 am

Haematocrit and Packed Cell Volumes

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Post by micco13 » Mon Oct 06, 2008 8:53 am

Hi there,

What is the significance of lower % PCVs or higher % PCVs in blood (mixed with saline solutions)? We did an experiment and there is a decreasing trend between PCV and NaCl ... Does the low PCV indicate that more cells has lysed (at the higher NaCl conc)? How would you tell which concentration of saline is isotonic?

Your help will be much appreciated

MrMistery · Post by **MrMistery** » Fri Oct 17, 2008 2:51 am

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Post by MrMistery » Fri Oct 17, 2008 2:51 am

0.9% is isotonic. And you would expect more cell lysis at lower concentrations...

"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

MichaelXY · Post by **MichaelXY** » Fri Oct 17, 2008 3:39 am

Re: Haematocrit and Packed Cell Volumes

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Post by MichaelXY » Fri Oct 17, 2008 3:39 am

How would you tell which concentration of saline is isotonic?

Cell appearence should give a clue.