Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
Moderators: honeev, Leonid, amiradm, BioTeam
-
ss_kalve
- Garter
- Posts: 35
- Joined: Sat Dec 08, 2007 8:07 am
Post
by ss_kalve » Sat Dec 15, 2007 8:42 am
Can we autoclave the blotting buffer?
-
canalon
- Inland Taipan
- Posts: 3909
- Joined: Thu Feb 03, 2005 2:46 pm
- Location: Canada
Post
by canalon » Sat Dec 15, 2007 6:56 pm
Which buffer? with composittion we could tell if some components are sensitive to heat.
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
-
ss_kalve
- Garter
- Posts: 35
- Joined: Sat Dec 08, 2007 8:07 am
Post
by ss_kalve » Mon Dec 17, 2007 4:28 am
tris base and glycine
-
canalon
- Inland Taipan
- Posts: 3909
- Joined: Thu Feb 03, 2005 2:46 pm
- Location: Canada
Post
by canalon » Tue Dec 18, 2007 3:00 am
As far as I know both can be autoclaved. But the question is : is it necessary to sterilize it?
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
-
ss_kalve
- Garter
- Posts: 35
- Joined: Sat Dec 08, 2007 8:07 am
Post
by ss_kalve » Wed Dec 19, 2007 4:58 am
I think so coz when i used autoclaved running buffer in electrophoresis it was giving good result than nonautoclaved one.so I'm thinking it should also give good result after autoclaving.
Who is online
Users browsing this forum: No registered users and 8 guests
