Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Helics
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by Helics » Sun Apr 06, 2014 4:07 pm
which is the best option for Restriction Endonuclease digesion ???
A) 37' C water bath
B) 37' C PCR program
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MOmarKhan
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by MOmarKhan » Mon Apr 07, 2014 2:09 am
Both of them are okay! But i would go for Thermo-cycler instead of water bath.
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Helics
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by Helics » Tue Apr 08, 2014 4:26 am
Dear scientist, Jack Bean I need your response.
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JackBean
- Inland Taipan
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by JackBean » Sun May 04, 2014 12:04 pm
thanks for considering me a scientist
I don't see much difference if you're sure that the temperature iscorrectly set. However, with thermocycler (or thermoblock) you do not have to worry, that you will spill your samples
http://www.biolib.cz/en/main/
Cis or trans? That's what matters.
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Javedz2001
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by Javedz2001 » Sun Oct 11, 2015 5:19 pm
Water bath is NOT recommended because after time the sample will evaporate and stick on the top of the cap. This will change the buffer composition. Always use PCR machine with heated lid or dry incubator.
Javed Siddiqi
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