Use of cDNA when studying splicing

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nukeman
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Use of cDNA when studying splicing

Post by nukeman » Wed May 15, 2013 11:33 am

Hi

I've read a few studies where the authors aim to study the association of certain proteins to mRNA in a splicing-dependent manner. Mainly they compared samples containing spliced mRNA with cDNA-samples, in a way that to me seemed as if they insinuated that the cDNA samples represented the non-spliced samples. cDNA is, as far as I know, made from mature mRNA which is fully spliced?

Could someone please explain this?

Thanks
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JackBean
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Post by JackBean » Thu May 16, 2013 9:42 am

can you show some such studies?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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DrGavinSee
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Re: Use of cDNA when studying splicing

Post by DrGavinSee » Mon Feb 16, 2015 7:06 am

cDNA is reverse transcribe from mature mRNA which only contains exons (coding regions) with introns (non-coding regions) remove. The non-coding region is removed by splicing leaving the exons to be transcribe into proteins. because the proteins are translated from mature mRNA, scientist would associate the protein of interest to the mRNA sequence and therefore reverse transcribing to the cDNA sequence to know the specific gene for that particular protein.
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