ANNTI FUNGI !

[Nmr]

ANNTI FUNGI !

• Quote

Post by Nmr » Sat Sep 17, 2011 2:14 pm

HI GUYS...

WHAT IS THE BEST ANTI-FUNGI THAT CAN I USE IN MY BACTERIAL CULTURE ?

COZ, I HAD FUNGI IN MY BACTERIAL BROTH MEDIUM !

THANK YOU

[JackBean]

• Quote

Post by JackBean » Sat Sep 17, 2011 2:27 pm

1) don't shout on us
2) I think that pH should be quite enough. Further you can use some antibiotic.

[canalon]

• Quote

Post by canalon » Mon Sep 19, 2011 7:18 pm

Or just be careful with your culture technique, ie if you did not put a sample that contained a mixed population of micro-organisms in your medium, what you need is proper aseptic techniques rather than anti-fungal. Just saying.

[choozi]

• Quote

Post by choozi » Sat Oct 22, 2011 3:26 pm

YOU CAN USE NYSTATIN OR AMPHOTERICIN-B AS ANTIFUNGAL.

[JorgeLobo]

Re: ANNTI FUNGI !

• Quote

Post by JorgeLobo » Sat Oct 22, 2011 6:18 pm

Agree with canalon. If you can;t maintain aseptic technique and pure cultures - get out of the micro lab.

I'll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.

[daniel.kurz]

Re: ANNTI FUNGI !

• Quote

Post by daniel.kurz » Mon Oct 24, 2011 12:46 am

Agree with canalon. If you can;t maintain aseptic technique and pure cultures - get out of the micro lab.

I'll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.

I agree both above. Use a flame and some ethanol and be careful. Anti-fungals can change microbioal physiology that is not natural and make your experiments not as valid. That grammar is terrible...