RNA interference
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RNA interference
In plants for getting a hairpin loop structure in pHannibal vector people normally use cDNA as the starting material from which to amplify the gene of interest. My question is whether one can start with the genomic DNA? What happens if introns are present in the region which will form ds RNA after transcription? The basic idea being that cDNA preparation can be avoided.
Hi,
there are several modes of RNA interference, one of them is the binding to DNA thus blocking transcription. Unfortunately, I don't remember, whether this is plant's or animal's case...
If it was possible, then using gDNA would be fine. If it worked classicaly through binding to mRNA and it's digest, then your RNAi would have non-complementar part and probably would not work
there are several modes of RNA interference, one of them is the binding to DNA thus blocking transcription. Unfortunately, I don't remember, whether this is plant's or animal's case...
If it was possible, then using gDNA would be fine. If it worked classicaly through binding to mRNA and it's digest, then your RNAi would have non-complementar part and probably would not work

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Cis or trans? That's what matters.
Cis or trans? That's what matters.
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