Enzyme kinetics

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
New forum link: https://forum.biologyonline.com/index/molecular-biology

Moderators: honeev, Leonid, amiradm, BioTeam

The new Biology Online has arrived!

We are very pleased to announce the website's new design and features. As a biology website pioneer in 2001, it has grown to become the world's most comprehensive online database for biology terms and topics. Thus, we would like to invite you to take part in this significant milestone by checking out these latest features from Biology Online.

Biology Dictionary

The largest online biology dictionary with over 70,000 biology terms! Learn various topics, concepts, and principles in biology. Start by searching here.

Biology Articles

Get the latest biology news, insights, trends and discoveries in biology and other related research fields. We have over 5,000 articles you can choose from.

Biology Tutorials

From the basics to the more advanced lessons, our tutorials will help you learn various topics in biology. Browse from over 100 free biology lessons here.

New Biology Forum

We hold one of the largest discussion boards in biology online! Join our community by signing up here or log in here if you already registered with us before.

Thank you and we hope you will join us as the site continues to grow by leaps and bounds.

Sincerely,
Biology Online Team

Locked
ManBearPig89
Garter
Garter
Posts: 28
Joined: Mon Mar 01, 2010 12:20 am

Enzyme kinetics

Post by ManBearPig89 » Sun May 30, 2010 11:28 am

Hello! I have a question about enzymes...

When looking at the [S] and comparing it to Vmax, does this mean that Vmax is the point when an enzyme can no longer take in any more substrate (moles/ secound?) in it's active site, and when an enzyme is "working hardest" ? I mean, this is when the reaction occours faster (if [S] is high) and slower when [S] is low...

Is the graph showing the rate (=speed?) of reaction in ONE enzyme, or in all the enzymes converting substrate to produckt?


Image

thank you!

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Sun May 30, 2010 4:36 pm

the graph is showing the reaction rate in your test system. there is definitely more than one enzyme molecule there.

at Vmax virtually all enzymes are occupied with substrate molecules. As soon as one molecule of substrate dissociates, another one binds
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Wed Jun 02, 2010 10:12 am

as MrMistery said, the speed is of all enzyme present in the system (well, theoretically, you could probably express it as speed of one enzyme molecule).

However, Vmax or Vlim is relevant only to the high S concentrations! When [S] is low, you have nothing to do with Vlim.
Also, you should actually never reach Vlim, that's why is it V limiting ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Locked