Difference between? Complementaton and Recombination
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Difference between? Complementaton and Recombination
In light of the Complementation test performed by Benzer on T4 phages, what is the difference between complementation and recombination?
(Can you have complementation and not have recombination, can you have complementation and have recombination?
(Can you have complementation and not have recombination, can you have complementation and have recombination?
I think, that complementation and recombination has nothing to do basically.
Recombination is exchange of genetic material between two DNA molecules (chromosomes, plasmids etc.), whereas complementation is process, when you add some gene to compensate for missing endogennous gene.
E.g. bacteria need some osmosensor to grow, so when you have knock-out of it, they are not able to survive (maybe only on osmotic media, not sure now), but when you add some other histidine kinase and it's signal, they are viable. Apparently, because the new HK supplemented bacteria with "osmosensor", but there does not have to be recombination (it can stay on plasmid).
Recombination is exchange of genetic material between two DNA molecules (chromosomes, plasmids etc.), whereas complementation is process, when you add some gene to compensate for missing endogennous gene.
E.g. bacteria need some osmosensor to grow, so when you have knock-out of it, they are not able to survive (maybe only on osmotic media, not sure now), but when you add some other histidine kinase and it's signal, they are viable. Apparently, because the new HK supplemented bacteria with "osmosensor", but there does not have to be recombination (it can stay on plasmid).
http://www.biolib.cz/en/main/
Cis or trans? That's what matters.
Cis or trans? That's what matters.
Re:
JackBean wrote:I think, that complementation and recombination has nothing to do basically.
Recombination is exchange of genetic material between two DNA molecules (chromosomes, plasmids etc.), whereas complementation is process, when you add some gene to compensate for missing endogennous gene.
E.g. bacteria need some osmosensor to grow, so when you have knock-out of it, they are not able to survive (maybe only on osmotic media, not sure now), but when you add some other histidine kinase and it's signal, they are viable. Apparently, because the new HK supplemented bacteria with "osmosensor", but there does not have to be recombination (it can stay on plasmid).
"How" did HK do this? Did you guess about the HK doing that? How did you prove(or how was it proven) that it was supplemented with the osmosensor"?
Re:
JackBean wrote:they are quite similar, so they can supplement each other. This system is commonly used in such situations.
There is system in both E.coli and Saccharomyces
it is the Ecoli system that was given as an example in class. Trying to understand that one. with the B strain K strain.
Re:
JackBean wrote:that is something different. I'm talking about histidine kinases, which work as receptors and thus, if you have knock-out of one of them, you can supplement it with plant histidine kinase
I went to my teacher yesterday and it seems I was remembering examples versus concepts. I though Ecoli was used as an example but it turns it out that is was specific to Ecoli and not to every system.
NOw trying to unlearn somethings and regroup my thoughts. Thanks for replying.
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