Amount of DNA for eficient transformation
Moderators: honeev, Leonid, amiradm, BioTeam
Amount of DNA for eficient transformation
Hello everybody. I was just wondering if there any formula to calculate the necessary amount of DNA for effective transformation. Maybe someone know where to look in to get this information. Thank you
Andrey
Andrey
Re: Amount of DNA for eficient transformation
not much at all is necessary. for example, commercially available vectors for transformation efficiency control are transformed at the pg level. however, if u are using ligation products, i used something like 50ng.
Re: Amount of DNA for eficient transformation
Thanks, I also use 50-100ng, but I was wondering maybe there is some formula to count.
Who is online
Users browsing this forum: No registered users and 19 guests