Amount of DNA for eficient transformation

Snoopy · Post by **Snoopy** » Wed Jan 28, 2009 8:11 am

Amount of DNA for eficient transformation

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Post by Snoopy » Wed Jan 28, 2009 8:11 am

Hello everybody. I was just wondering if there any formula to calculate the necessary amount of DNA for effective transformation. Maybe someone know where to look in to get this information. Thank you

Andrey

pcrboy · Post by **pcrboy** » Thu Jan 29, 2009 3:30 am

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Post by pcrboy » Thu Jan 29, 2009 3:30 am

you mean transfection? or cancer transformation?

Snoopy · Post by **Snoopy** » Thu Jan 29, 2009 7:45 am

Re:

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Post by Snoopy » Thu Jan 29, 2009 7:45 am

pcrboy wrote:you mean transfection? or cancer transformation?

I mean, transformation - foreign DNA to host cell.

pcrboy · Post by **pcrboy** » Thu Jan 29, 2009 12:10 pm

Re: Amount of DNA for eficient transformation

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Post by pcrboy » Thu Jan 29, 2009 12:10 pm

not much at all is necessary. for example, commercially available vectors for transformation efficiency control are transformed at the pg level. however, if u are using ligation products, i used something like 50ng.

Snoopy · Post by **Snoopy** » Thu Jan 29, 2009 12:13 pm

Re: Amount of DNA for eficient transformation

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Post by Snoopy » Thu Jan 29, 2009 12:13 pm

Thanks, I also use 50-100ng, but I was wondering maybe there is some formula to count.