PCR question
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PCR question
Why is there a need for PCR when there is helicase-dependent amplification??????
In http://en.wikipedia.org/wiki/Helicase-d ... lification, wikipedia states that HDA takes longer than PCR. My question is WHY?????? [Even though the helicase might unwound it slowly (as evident in DNA replication) it's not as if the DNA polymerase can elongate the growing DNA strand any faster (again refer to DNA replication), right?????]
In http://en.wikipedia.org/wiki/Helicase-d ... lification, wikipedia states that HDA takes longer than PCR. My question is WHY?????? [Even though the helicase might unwound it slowly (as evident in DNA replication) it's not as if the DNA polymerase can elongate the growing DNA strand any faster (again refer to DNA replication), right?????]
That wiki article was probably posted by someone from the kit manufacturer. You probably should consider it a bit of advertising. I don't know anyone using this alternative to pcr. The only "advantage" I can see is the ability to run everything at one temperature, and I don't see that as a great plus. If the method was obviously superior to pcr it would be widely used already. I suspect it does not work as well as advertised.
http://www.nature.com/embor/journal/v5/ ... 00200.html
lacks refinement, doesn't work on long seqs, but it's interesting enough to be in nature
lacks refinement, doesn't work on long seqs, but it's interesting enough to be in nature
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Accepting hardships as the pathway to peace;
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Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
The article lies. PCR does denature DNA, at around 95C. So therefore it must become unwound for the two strands to separate. Never use wikipedia as an academic resource, it's too untrustworthy and contrary to what some people say, it is not fully protected. I know from experience, it can be very hard to keep correct info on a page and permanently rid it of nonsense, as someone can easily vandalise it again.
Re:
PCR is around 4 hours max if you use the maximum number of cycles (40) and normally 3 hours long. Surely 3 - 4 hours isn't too long to wait. Inbetween you can get on with other lab work.thewax wrote:Thanks!!!!!!
But why wouldn't it????
I mean, compare the helicase and DNA polymerase to those used in DNA replication (they are the same, and in DNA replication, they move really fast), right?????????
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