glucose transporters
Moderators: honeev, Leonid, amiradm, BioTeam
-
- Coral
- Posts: 260
- Joined: Tue Mar 21, 2006 9:33 am
glucose transporters
Hi there,
so I have to write up a report like thing on glucose transporters and how "to find expression of various glucose transporter isoforms in the cortex at the mRNA level"!!!...and we have to talk about what steps to take etcetc...umm have NOOO idea where to begin other than say use trizol to extract RNA from brain??...and isolate mRNA...buuut how to tell between the diff. isoforms...any help in terms of what i should be looking up online/articles/key words would be muuuch appreciated...
cheers
b_06er
so I have to write up a report like thing on glucose transporters and how "to find expression of various glucose transporter isoforms in the cortex at the mRNA level"!!!...and we have to talk about what steps to take etcetc...umm have NOOO idea where to begin other than say use trizol to extract RNA from brain??...and isolate mRNA...buuut how to tell between the diff. isoforms...any help in terms of what i should be looking up online/articles/key words would be muuuch appreciated...
cheers
b_06er
au contraire
From wikipedia excerpt
These molecules are used as probes to measure the relative abundance of the complementary sequences present in the samples (target) under study. This is done by hybridizing the two under high-stringency conditions and then detecting the sequences of the sample, which consist of a mix of either cRNA or cDNA commonly fluorescently labelled.
You can measure the intensity of the fluroescence with lasers.
From wikipedia excerpt
These molecules are used as probes to measure the relative abundance of the complementary sequences present in the samples (target) under study. This is done by hybridizing the two under high-stringency conditions and then detecting the sequences of the sample, which consist of a mix of either cRNA or cDNA commonly fluorescently labelled.
You can measure the intensity of the fluroescence with lasers.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
Microarrays are quantitative: Products are labeled during or after RT and since the intensity of the signal depends on the number of attached molecules. you can read deduce the relative expression of a gene compared to a standard (housekeeping gene usually) just as well as with qPCR.
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
-
- Coral
- Posts: 260
- Joined: Tue Mar 21, 2006 9:33 am
- MrMistery
- Inland Taipan
- Posts: 6832
- Joined: Thu Mar 03, 2005 10:18 pm
- Location: Romania(small and unimportant country)
- Contact:
Cost would also be involved: where I work we don't afford microarrays(which was the main reason I didn't know exactly how a microarray works - I have never actually seen one). And I'm thinking the number of genes involved: if you want to study only 2 or 3 genes(as might be the case with your glucose transporters) Real time would work just fine. If you want more genes, using a DNA chip would save you a ton of time, since you can analyze a truck-load of genes at once.
But i'm really just guessing here. I'm not very familiar with either
But i'm really just guessing here. I'm not very familiar with either
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
Who is online
Users browsing this forum: No registered users and 12 guests