Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Death Adder
- Posts: 98
- Joined: Tue Jun 28, 2005 7:41 am
- Location: India
Restriction enzymes like endonuclease, ECO R1 cut DNA at particular sequences like GAATTC ..
Then this DNA fragment can be inserted to a plasmid which can be transfered to a vector( bacteria ) to multiply it and to produce a gene library.
- King Cobra
- Posts: 586
- Joined: Sat Jul 30, 2005 7:16 pm
- Location: holland
usually they cleave DNA at so called palindromes. Recall that besides cohesive ends, blunt ends can also be generated, which are more difficult to ligate in a plasmid.
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