## Plasmid Restriction Maps

**Moderators:** honeev, Leonid, amiradm, BioTeam

### Plasmid Restriction Maps

I'm having problem drawing a plasmid map with the following combination enzymes...

BamH1: 900/1500/3000

Hindlll: 500/1600/3300

BamH1 + Hindlll: 300/400/500/1200/3000

Is there a simple way to solve this?

BamH1: 900/1500/3000

Hindlll: 500/1600/3300

BamH1 + Hindlll: 300/400/500/1200/3000

Is there a simple way to solve this?

see the no. of frags = no. of sites

so first and second both have 3 sites each, but the combined case has not 3+3=6 but 5, so they sahre one site...

now the combined result can be treated as one obtained by two stepped restriction...i mean imagine 1st BamH1 gives u 3 frags...

now u treate these 3 frags with the Hindlll, so u get the above said 5 frags ,

so u need to cut those only-BamH1 frags into those 5 frags...

you can form only smaller or equal length frags from them, so u get this constiant,

here Bam1 gives us 3000,1500,900

and the combined treate gives 300,400,500,1200,3000

now the 3000 seems remain intact,

while 900 frag seems get broke into 300, and 400 frags

and the 1500 frag into the 1200 and 300 strands

now u get the possible distances between BamH1 and Hindlll sites...

now try to make out the only Hind result from this to narrow down the possible plasmid map possibilities...

or

from combined result try to make the 'only' results by associating diff. strands togather...this is faster,

to get Hindlll result

500 is intact, 400 and 1200 associate, 300+3000 associate

so the sequence is -[B/H]500[H] 400 [B] 1200 [H] 300[B] 3000-

[B]= BamH1 site

[H]= HIndlll site

[B/H]= both

it ws nice exercise, thanks...

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