Pericentromere specific probes for FISH

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Pericentromere specific probes for FISH

Post by Matan666 » Wed May 18, 2016 2:53 pm

Hi all,

Im trying to do a FISH on DNA combing for pericentromeric regions.

As you may know, the human centromere is actually separated into two distinct regions: 1) centromeric region - which is involved in kinetochore binding 2) pericentromere region - a heterochromatic region which is involved in sister chromatid binding.

Im looking for pericentromere specific probes which do not overlap with any other region (centromeric or non centromeric).

Does anyone have a clue whether this kind of probes/BAC/HAC/anything else exist? if so - a link would be great!

Thank you!


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