Minimum Data Points for elisa standard curve

[rgm]

Minimum Data Points for elisa standard curve

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Post by rgm » Sun Feb 01, 2015 7:19 am

Hi

I have two questions:

1. In my Elisa results, I have 4 standard calibrators and 1 negative standard (conc= 0 IU/ml).Is it practical to use 4PL or 5PL equations for only 5 data points( 5 standards)?

2. In Elisa test instruction that the company gave me, it is noted that the calibration curve should be a linear plot. But my results can be better explained by non linear curves. Am I allowed to disregard test instruction in this case?

Any help would be appreciated.