Stem Cells

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Shaanu
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Stem Cells

Post by Shaanu » Fri May 10, 2013 8:10 am

How can one prove in vivo that a cell is a stem cell ? Can anyone give hints citing any two methods.
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JackBean
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Post by JackBean » Fri May 10, 2013 9:34 am

my answer would be probably gene expression profiling and differentiation assay (if there is any)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Shaanu
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Re: Stem Cells

Post by Shaanu » Fri May 10, 2013 1:59 pm

Thanks !
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JackBean
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Post by JackBean » Fri May 10, 2013 3:54 pm

I'm not saying that's right answer ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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biohazard
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Post by biohazard » Tue May 14, 2013 6:56 am

For certain stems cells, there are cell surface markers (cluster of differentiation; CD) that can also be used to track down these cells, either with flow cytometry or other monoclonal antibody/fluorescence-based assays. The problem is that the stem cells are usually very rare and thus difficult to detect, and in many cases the CD markers are not well defined. But if you have a cell and want to know if it is a stem cell, then you can try staining it with fluorochrome-labeled antibodies for certain CD markers and check it out e.g. with a confocal laser microscope. Or if you have many cells, run them through a flow cytometer.
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