How can I add a kozak sequence in plasmid pMT/bip/V5-His A

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How can I add a kozak sequence in plasmid pMT/bip/V5-His A

Post by yiwi123 » Tue Mar 12, 2013 8:04 pm

Hi guys,

I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? So where should I add the Kozak sequence?

Thanks for your time.

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Post by JackBean » Wed Mar 13, 2013 4:59 pm

the Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life Technologies.

Cis or trans? That's what matters.

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