Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Post by siddharthsameer » Sun Aug 12, 2012 5:10 pm

hello all
I would like to ask,is that i already of the used up my vector, and I plated the vector on one of the lb plate which i used as a negative control at the time of doing ligation,,So i would be trying to make the preculture from that and then the main culture and would do the miniprep to get my vector..I can easily know the concentration through UV but how do I get the mass of my vector...My vector is pPICZalphaA ....
since i am doing all this thing for the first time, so kindly help me.....

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Post by triton » Wed Aug 22, 2012 7:34 pm

I dont get it. What do you mean by MASS??? If the total amount i.e. weight, so simply multiply your concentration by the total volume...

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Re: Vector

Post by citroenboom » Mon Sep 03, 2012 11:36 am

Maybe check the marker? Then you will find the amount of basepairs.

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