restriction analysis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Post by citroenboom » Tue Jul 17, 2012 9:31 am

If your PCR is successful (check 5 ul on 0.8% agarose after the run) you can load the whole PCR mix on gel and cut it out. I prefer to use PCR-purification kit, but both works. So, yes, I think your procedure should work fine.

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Post by JackBean » Tue Jul 17, 2012 10:32 am

sid: That's depends, whether is the PCR only to confirm that you have the gene cloned into vector or whether you want to do with the PCR amplicon something subsequently. I would bet on the first option, because pPICZ is expression vector.

Cis or trans? That's what matters.

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Re: restriction analysis

Post by siddharthsameer » Tue Jul 17, 2012 1:31 pm

I am about to start and my first step would be the amplification of my desired gene.. and then digestiona nd ligation into expression vector.. so If i start from the first step... thanks you so mucg for clearing my doubts...

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