GFP fusion protein not expressing!

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GFP fusion protein not expressing!

Post by biology_06er » Wed Jun 27, 2012 10:19 pm

Hi there,

I tried to make a GFP fusion protein (my gene of interest was fused to the C terminal of GFP) but no expression occured...I tested the expression of the two proteins alone and individually they worked but not when fused together..I tried a linker between the two..still no luck...could it just be that the fusion protein is somewhat toxic to the cell (doesn't seem likely aye..especially if the 2 alones are ok)



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Inland Taipan
Inland Taipan
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Post by JackBean » Thu Jun 28, 2012 9:35 am

Try the other side of the protein or other tag. Sometimes it just doesn't work.

Cis or trans? That's what matters.

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