Restriction mapping

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Restriction mapping

Post by moonj88 » Sat Mar 19, 2011 3:36 am

Please help me identifying with problems and solutions for situations in which,

1) DNA fragments are too large
2) DNA fragments comigrate on the gel
3) DNA fragments are too small.

Thank you..!

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Inland Taipan
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Post by JackBean » Sun Mar 20, 2011 11:01 am

1) you won't be able to distinguish between two pieces, which may have quite different sizes, but are so big, that they seem to be one. Moreover, the resolution is quite bad, so you can make a mistake of even 500 or 1000 bp
2) you will think there is only one fragment and thus have a mistake
3) they can run out of gel or due to their small size they may not be visible on the gel (in comparison with the other fragments)

Cis or trans? That's what matters.

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Inland Taipan
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Post by canalon » Mon Mar 21, 2011 11:13 pm

Already answered to 2 and 3 somewher else. For 1 use a different type of migration. I suggest PFGE.

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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