HELP!!bacterial trasformation

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agluke
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HELP!!bacterial trasformation

Post by agluke » Fri Mar 18, 2011 10:53 am

Hi!
I have trasformed DH5alpha competent bacteria with a plasmid, and after the heat shock I resuspended them in YPD instead LB!!! Will it work anyway??

canalon
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Post by canalon » Fri Mar 18, 2011 11:39 am

Gosh Darn it!
You wanted tuse LB? All protocols I have ever done use SOC which is richer. Your transformation is going to the weeds my friend. Or not.
You might have decreased a little bit the efficiency, but YPD is quite the rich medium anyway, so I guess they should recover nicely. Do not worry.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

JackBean
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Post by JackBean » Mon Mar 28, 2011 10:10 am

:lol: saved an experiment by mistake :lol:
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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