Lambda DNA stuck in well

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Lambda DNA stuck in well

Post by NiceOnTheIce » Sun Nov 28, 2010 6:31 pm

Hi there,
Why might my uncut lambda DNA have gotten stuck in my agarose gel well? When I had the gel visualized and a picture printed of my fluorescing bands, I noticed that my uncut lambda DNA had not moved from the well, except for a light band that did not even travel as far as the band depicting the longest molecules in my DNA ladder (10.0 Kb). I might have loaded the gel incorrectly, however, the other lanes look normal, and I was very careful, so I don't really think that's why. Are there other possibilities?

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Post by JackBean » Mon Nov 29, 2010 6:21 am

Cis or trans? That's what matters.

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Post by canalon » Mon Nov 29, 2010 4:55 pm

Or in short because you have actually managed to be very careful and not to break your Lambda gDNA. Congrats.
Large molecules do not move very well in regular electrophoresis. PFGE would be useful in your case.

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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