Hot start pcr

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 1
Joined: Fri Jul 30, 2010 12:41 am

Hot start pcr

Post by ziaurahman » Wed Aug 04, 2010 1:10 am

hello every body,

I am in intense need of the protocol for hot start pcr, if any one knows all steps , please write here or give the reference

thanks in advance

Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Wed Aug 04, 2010 5:00 am

I think the protocol is pretty much the same as for the regular PCR. Look into the paper you got with the Pol

Cis or trans? That's what matters.

Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Wed Aug 04, 2010 10:07 pm

If you do not have a special hot start polymerase (in wax beads, attached to antibodies or any other technique) you simply add a pause after the initial denaturation at the first step of your PCR and add the polymerase manually at that moment and then start the cycling as usual. Nothing very complicated.

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests