plasmid preparation

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plasmid preparation

Post by buthercup » Tue Aug 03, 2010 6:57 pm

Hi everyone,
I'm working with Gram-negative bacteria, trying to charactirize some strains isolated form nature. After succesfull DNA extraction with a comertial kit (GeneElute Bacterial Genomic DNA, Sigma), does anyone know how can I verify if the genomic DNA (total DNA) preparation contains also plasmids?
I was thinking in start to try differents plasmid extraction kits, but I need to know first if I really have something to extract, and I need to have an idea of it size (HCN, LCN).
I herad something about a special electrophoresis to observe all differents replicons presents in bacteria. Does any one know a detailled protocole?

Thankyou in advance

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Inland Taipan
Inland Taipan
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Post by JackBean » Wed Aug 04, 2010 5:08 am

look for pulse field electrophoresis. That is able to resolve large pieces of DNA.

Cis or trans? That's what matters.

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