tyrosine solution

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
piefke
Garter
Garter
Posts: 24
Joined: Tue Sep 18, 2007 8:41 am

tyrosine solution

Post by piefke » Fri May 21, 2010 9:42 am

Hi

I want to do an enzyme assay in EPPS buffer pH= 7. I want to test the effect of tyrosine on the activity of my enzyme and therefore I have to add tyrosine in different concentrations to the buffer (end concentration
in 100 µl assay 10 - 250 µM).
The problem is: Tyrosine is not very soluble in water or in the buffer. I can´t solve it in HCl because my assay conditions then get acidic. Do you have any idea or experiences in working with amino acids?

JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Fri May 21, 2010 10:17 am

what is the solubility of Tyr in water? You could make stock solutions in DMSO. I think, that 250 uM should be soluble, shouldn't it?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

piefke
Garter
Garter
Posts: 24
Joined: Tue Sep 18, 2007 8:41 am

Post by piefke » Fri May 21, 2010 10:45 am

But don´t you think DMSO will disturb the activity of my enzyme?

JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Fri May 21, 2010 12:36 pm

that depends on how much will you put in of course ;) We're typically adding 10 ul of substrate into 500 ul reaction. If you measure spectoscopically, you have to measure against blank, which contains DMSO of course ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Post Reply

Who is online

Users browsing this forum: No registered users and 9 guests