Horseradish Peroxidase protein does not SHow up on PAGE gel

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Horseradish Peroxidase protein does not SHow up on PAGE gel

Post by AlaRoshdieh » Thu May 20, 2010 2:50 am

Okay, i ran HRP with polyacrylamide gel electrophoresis, the buffer that i used to store the HRP is SOdium phosphate monohydrate and it worked out alright. I loaded the wells with beta-mercaptoethanol and all the proteins had sodium dodecyl sulphate (SDS), all my proteins ran succesfuly on the gel except HRP which was NON-EXISTENT after i destained all the coomasie blue. Are there any reasons to why this happened?

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Post by JackBean » Sat Jun 12, 2010 5:56 pm

I don't think that there is any reason, why shouldn't be one protein visible if is it present in sufficient amount...

Cis or trans? That's what matters.

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Post by canalon » Sun Jun 13, 2010 2:53 am

How did you test for the presence of HRP and of the other proteins?

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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