Dissociation Constants

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Dissociation Constants

Post by pmngrad » Sun May 16, 2010 7:06 pm

Quick question of clarification,

If a particular receptor has a dissociation constant (Kd) of 50nM for instance. That means that the receptor is half bound by ligand when the concentration of the ligand is 50nM.

To fully saturate a receptor, would one use twice as much ligand? 100nM in this case?

Thanks for your help with this....

All the best :)

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Inland Taipan
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Post by JackBean » Sun May 16, 2010 8:00 pm

not really, it's similar as Michaelis with enzymes, so you won't get twice so much bound ligands ;)

Usually, 10× more is taken to fully saturate ;)

Cis or trans? That's what matters.

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