Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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I'm treating TF-1 cells with forskolin and measuring cAMP increase by ELISA, but I have not been able to see a significant increase with varying concentrations of forskolin. I've tried multiple treatment volumes and concentrations, and I'm using cell count dictated by the ELISA. 1x10^7 cells/mL. Has anyone else had this trouble in TF-1s or does anyone have any helpful tips? Thanks.
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