Ligation question.
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Ligation question.
Hey guys,
I am currently in a 3rd year molecular lab and I am conducting my first ligation next week.
I already digested my fragment of interest however I made a silly error.
For my ligation, I am also required to run a gel for the fragment of interest. I was suppose to set aside 1.5 microlitres of DNA with loading dye in a separate tube, where it will be ran on a gel. The remaining solution of DNA is the DNA I am going to ligate.
HOWEVER, I am an idiot and put the loading dye to all my DNA, even the DNA I am suppose to ligate.
Will the dye effect my ligation process?
like intuitively I would say that it wouldn't effect the ligation process... it doesn't really react with DNA, that is why it is safe to add to DNA when we run it on a gel.
However, has any one done this before? lol...
what's your insight? do you think my ligation will go fine?
I am currently in a 3rd year molecular lab and I am conducting my first ligation next week.
I already digested my fragment of interest however I made a silly error.
For my ligation, I am also required to run a gel for the fragment of interest. I was suppose to set aside 1.5 microlitres of DNA with loading dye in a separate tube, where it will be ran on a gel. The remaining solution of DNA is the DNA I am going to ligate.
HOWEVER, I am an idiot and put the loading dye to all my DNA, even the DNA I am suppose to ligate.
Will the dye effect my ligation process?
like intuitively I would say that it wouldn't effect the ligation process... it doesn't really react with DNA, that is why it is safe to add to DNA when we run it on a gel.
However, has any one done this before? lol...
what's your insight? do you think my ligation will go fine?
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