linear plasmids and pulse field elfo

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JackBean
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linear plasmids and pulse field elfo

Post by JackBean » Thu Nov 19, 2009 4:50 am

Hi,
I just found one article about some linear plasmid in one phytopatogenic bacteria. So far, I thought, that plasmids are by definition circular? Do you anybody know, whether such plasmids have some telomeres as well?

Also, it was mentioned in the article, that it's probably linear, because it moves in pulse field electrophoresis (at least I understood so). Does that mean, that PFE does not work for circular DNA?
http://www.biolib.cz/en/main/

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zami'87.
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Re: linear plasmids and pulse field elfo

Post by zami'87. » Thu Nov 19, 2009 11:39 am

Well I know that some bacteria have linear chromosomes(eg Borrelia burgdorferi)..
http://microbewiki.kenyon.edu/index.php/Borrelia
Probably such plasmids exist too..
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