Real-time PCR&Reverse transcription PCR

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 1
Joined: Wed Nov 04, 2009 7:56 am

Real-time PCR&Reverse transcription PCR

Post by Elva » Thu Nov 05, 2009 2:41 am

Such as subject ,when we see qRT-PCR,what is the really meaning of "RT"?
What are the difference between them? :)

User avatar
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN

Post by mith » Thu Nov 05, 2009 4:40 am

look it up
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;

User avatar
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Thu Nov 05, 2009 4:47 am

RT can be both reverse transcriptase as well as real-time ;)

Cis or trans? That's what matters.

User avatar
Posts: 20
Joined: Sat Oct 31, 2009 2:01 pm

Post by qqsvery » Thu Nov 05, 2009 12:59 pm

Real-time PCR, also known as kinetic PCR, qPCR, qRT-PCR and RT-qPCR, is quantitative PCR method for the determination of cope number of PCR templates such as DNA or cDNA in a PCR reaction. There are two flavors of real-time PCR: probe-based and intercalator-based.

I`m sure you will be interesting to look into this website to learn more about it!Here it is: ... index.html
A Strong Man Can Save Himself A Great Man Can Save Another.

User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Thu Nov 05, 2009 2:25 pm

To sum everything up, due to the 2 different possible meaning of RT-PCR, people are gradually shifting to q for quantitative, instead of Real-Time. So it becomes clearer to describe what is done:
qPCR: quantification of DNA
RT-PCR: amplification of RNA
qRT-PCR: quantification of RNA

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 12 guests