DNA polymerase I question

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 170
Joined: Wed Mar 05, 2008 3:09 am

DNA polymerase I question

Post by Darwin420 » Fri Oct 16, 2009 9:16 pm

Hey guys...I am really stuck on this problem question...wondering if anyone could help me out.

Is make of RNA primers (by primase), which have to be removed and replaced with dNTP's (by DNA polymerase I) actually wasteful and energetically inefficient process?

I would say no considering how else is DNA polymerase I going to fill in the gaps of the okazaki fragments?

I really don't know how to answer this question...

User avatar
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Fri Oct 16, 2009 10:56 pm

I don't think, this has anything to do with Okazaki fragments.

The point is, that DNA Pol needs to continue on some strain, it can't just start from nothing, that's, why there are primers ;)
And their removal is due to no proof-reading capability of the starting enzyme (no matter, whether RNA or DNA polymerase), so in this piece there can be some mutations

Cis or trans? That's what matters.

User avatar
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)

Post by MrMistery » Sat Oct 17, 2009 1:34 am

it is pretty energetically inefficient, but it's all nature could come up with. Do you have anything better?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Post Reply

Who is online

Users browsing this forum: No registered users and 31 guests