nested pcr
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nested pcr
How to get rid of larger non-specific bands appearing in 1st round of pcr just above the expected bands (of approx. 1.5 kb)? The non-specific bands from the 1st pcr are amplified in 2nd round of pcr using nested pair of primer.
I would try to optimize the first rund of PCR. Tips and tricks to do thta can be found here:
http://info.med.yale.edu/genetics/ward/tavi/Guide.html
http://info.med.yale.edu/genetics/ward/tavi/Guide.html
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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