Electroporation of human bladder carcinoma 5637 cell line

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roniadam
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Electroporation of human bladder carcinoma 5637 cell line

Post by roniadam » Tue Oct 06, 2009 5:25 am

Hi Guys,

I am searching for the protocol of human bladder carcinoma 5637 cell line using electroporation technique. I mean what voltage, pulses and cuvette should I use for susccessful transfection? I use Genepulser machine from BioRad.
Any help please?
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JackBean
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Post by JackBean » Tue Oct 06, 2009 2:06 pm

Aren't you following some paper?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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roniadam
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Post by roniadam » Wed Oct 07, 2009 5:28 am

Hi JackBean:

I was not able to find any paper explaining the electroporation protocol for 5637 cells. all papers I saw are transfecting the 5637 using Lipofectin or Fugene. but no paper found explaining the electroporation ptotocol.
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JackBean
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Post by JackBean » Wed Oct 07, 2009 6:21 am

And you do not have any protocol from manufacturer? Are these cells electrocompetive? (able to perform electroporation)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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