RNA from feathers

[blurcita7]

RNA from feathers

• Quote

Post by blurcita7 » Tue Jul 21, 2009 3:33 pm

Hello,

Hope this post has a better outcome than my previous one which is still waiting for an answer! Do someone now how to extract RNA from feathers of adult birds? if so, can you suggest me some papers or methodologies to follow please?
Many thanks in advance

B

[MrMistery]

• Quote

Post by MrMistery » Sat Jul 25, 2009 12:24 am

here's something that sounds kinda like how you're looking for http://www.ncbi.nlm.nih.gov.ezp-prod1.h ... ed/4519025 but I have no clue how to get that paper, that journal has no website

[canalon]

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Post by canalon » Sat Jul 25, 2009 1:28 am

Hey Andrew you page is behind a wall (special version of NCBI for Harvard, did not know that existed...) the link is:
http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=4519025
But it seems that you have quite a choice if you type RNA Isolation from feather in Google. Like that:
http://jvdi.org/cgi/reprint/13/2/162.pdf
and I am pretty sure that this method can be adapted for RNA (use the lysis conditions then do a trizol purification instead of the phenol choloroform):
http://jvdi.org/cgi/reprint/13/2/162.pdf

[MrMistery]

• Quote

Post by MrMistery » Sat Jul 25, 2009 3:11 pm

oops, sorry about that. That thing they have from both pubmed and google scholar gets me instant access to full text of every paper, that's why I use that. I usually remember to give the proper link, but that one slipped. Sorry