why does taq add an "A"

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
theendperson
Garter
Garter
Posts: 2
Joined: Sat Mar 28, 2009 11:39 am

why does taq add an "A"

Post by theendperson » Sat Mar 28, 2009 11:43 am

Hiya I wanted to know why taq polymerase add an A to the end of a transcript

Thanks in advance

Tom

plasmodesmata11
Coral
Coral
Posts: 198
Joined: Sun Nov 30, 2008 3:51 am
Location: University of Rhode Island

Post by plasmodesmata11 » Mon Mar 30, 2009 12:15 am

is that something to do with a poly adenyl tail?

theendperson
Garter
Garter
Posts: 2
Joined: Sat Mar 28, 2009 11:39 am

Re: why does taq add an "A"

Post by theendperson » Mon Mar 30, 2009 8:27 am

dont think so, as far as I can tell it only adds 1 A

canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Mon Mar 30, 2009 3:43 pm

I don't know why. But it is very convenient for TA cloning. And it seems to be a unique chareacteristic of the Taq polymerase, others like Pfu do not do that. I have never seen any explanation for the why.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Tue Mar 31, 2009 3:34 am

I really doubt it actually has a role, honestly. I think it's much more probable that it's simply a result of drift in evolution... But this is just my opinion
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests