Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Yes you can use the mRNA sequence to design gDNA primers. If the primers happen to span an intron (one on each side of the intron) the product from gDNA will be larger then from mRNA and if the intron is very big you might not be able to span its length by PCR. I use Primer3 (http://fokker.wi.mit.edu/cgi-bin/primer ... r3_www.cgi) for designing primers.
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