IgG antibodies

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Joined: Wed Feb 06, 2008 2:07 am

IgG antibodies

Post by SmileyScience » Wed Feb 06, 2008 2:11 am

Hi All,

I need to use one of these three antibodies to detect TUBA1 protein in HUVEC cells using western blots:

TUBA1 isotype IgG2b
TUBA1 isotype IgG2a
TUBA1 isotype IgG1

Does anyone know what the main difference between the three IgGs is and which one would you recommend to use?


Smiley Science

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Joined: Fri Mar 30, 2007 4:23 am

Re: IgG antibodies

Post by blcr11 » Fri Feb 08, 2008 4:18 pm

I wouldn't have thought that the antibody isotype would make that much of a difference, but I don't do these things, so I may not know what I'm talking about.

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