Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Inland Taipan
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For Lineweaver-Burke plots the x-intercept is -1/Km, the y-intercept is 1/Vmax, and the slope is Km/Vmax. You can also do Eadie-Hofstee plots (v = -Km(v/[S]) + Vmax) in which case the x-intercept is Vmax/Km, the y-intercept is Vmax, and the slope is –Km. Lineweaver-Burke plots are more commonly used, though the Eadie-Hofstee plots sometimes give more accurate estimates of the parameters.
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