Best way to induce several mutations?

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Ammie
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Best way to induce several mutations?

Post by Ammie » Mon Sep 17, 2007 9:24 am

I have my insert in a topo plasmid and I want to change about five of the nt's. What way is the best you think?

canalon
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Post by canalon » Mon Sep 17, 2007 5:23 pm

It really depends where you want your mutations. Changing 5 successive (or very close) nucleotides and 5 nt spread over 1 kb are quite different things that will probably require different approaches.
Patrick

Science has proof without any certainty. Creationists have certainty without
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Ammie
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Post by Ammie » Tue Oct 02, 2007 3:05 pm

I had 5 that were spread over around 6000kb.

I decided to go with Multi QuikChange from Stratagene

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