DNA extraction of animal tissue
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- Garter
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DNA extraction of animal tissue
i ever used Intelligene's lysis buffer. Its' protocol is very easy. you can put your sample tissue in lysis buffer -->grind it well-->incubate at 95 degree celcius for 15 min-->centrifuge-->pipette the supernatant to 95% ethanol-->centrifuge-->dry pellet and dissolve with TE buffer or DI water.
After i check DNA quality with spectrophotometry and gel electrophoresis, i's ok-->there are protein interfere a little. I would like to know what is the component of this lysis buffer. Who can guess? please tell me.thank you.
After i check DNA quality with spectrophotometry and gel electrophoresis, i's ok-->there are protein interfere a little. I would like to know what is the component of this lysis buffer. Who can guess? please tell me.thank you.

I suspect more than one component. You can find some indication in the MSDS or safety sheets (in case you drink it you need to know what to do before you decide to extract your total DNA.
But you will probably find a strong detergent, a protein denaturant (GITC for example) and maybe something that will precipitate sugar such as CTAB.
But you will probably find a strong detergent, a protein denaturant (GITC for example) and maybe something that will precipitate sugar such as CTAB.
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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- Garter
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