DNA/RNA extraction

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DNA/RNA extraction

Post by arm_chair27 » Mon Mar 26, 2007 12:57 pm

what's different between using 95%ethanol and isopropanol in DNA/RNA extraction?

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Post by canalon » Mon Mar 26, 2007 4:20 pm

Isopropanol is slightly better at precipitating DNA (and butanol even better). It does need to be frozen or even a long incubation. And it might even be cheaper tha ethanol in many countries were alcohol is heavily taxed.

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