Search found 2072 matches

by victor
Wed Jul 16, 2008 1:53 am
Forum: Molecular Biology
Topic: gel extraction problem
Replies: 4
Views: 3989

Maybe the time is not long enough when you incubate the washed DNA with eluent buffer. In my lab, most staffs here use QiaGen QiaQuick Column and mostly they succeed in DNA extraction from gel.
by victor
Tue Jul 08, 2008 5:52 am
Forum: General Discussion
Topic: Spermicide vs Spermaticide
Replies: 5
Views: 4653

yeah, talking about improper word adoptions, I remember reading a translated book which originally titled 'Plant Physiology' by Salisbury & Ross. I still remember that the phrase 'red-drop effect' in photosynthesis is translated literally become 'efek tetesan merah'. Because: red = merah; drop =...
by victor
Tue Jul 08, 2008 2:07 am
Forum: Microbiology
Topic: Question about actinomyces
Replies: 7
Views: 6032

Yup, I've seen that and I'm stuck in front of the paywall...
hmm....so surprising that the antibiotic-consuming bacteria were closely related to human pathogen....
by victor
Thu Jul 03, 2008 6:43 am
Forum: Microbiology
Topic: Question about actinomyces
Replies: 7
Views: 6032

is there any link to get that paper? maybe I can read it to update my info :mrgreen:
by victor
Thu Jul 03, 2008 2:33 am
Forum: Human Biology
Topic: I have a question on glucose here!
Replies: 8
Views: 5610

glucagon will affect the phosphylation of glycogen through glycogen phosphorylase, thus releasing glucose-1P. This G1P then converted into G6P and enter glycolysis (if the molecule isn't transported via bloodstream); but if it's used to be transported to another tissue (ex maintaining the normal blo...
by victor
Thu Jul 03, 2008 1:44 am
Forum: Molecular Biology
Topic: Base change in genes/ mutations
Replies: 3
Views: 3009

Well, I can say that it happens in a specified exon which they'd make their own sequence (and the mutation happens in nucleotide 811 from T into C in their own numbering version). In the case, it's mentioned that the mutation yields an amino acid change (misense mutation). So, this mutation happens ...
by victor
Thu Jul 03, 2008 1:15 am
Forum: Microbiology
Topic: Question about actinomyces
Replies: 7
Views: 6032

hmm...carbon source is able to get from antibiotics degradation, but for energy sources I don't think that antibiotics provide it.
by victor
Thu Jul 03, 2008 12:51 am
Forum: Cell Biology
Topic: Biochemistry help?
Replies: 3
Views: 7812

Yup, you can see whether the process is exergonic or endergonic by calculating the free Gibbs energy. If the calculation yields a negative result, th the process is exergonic and vice versa. As far as I know that lactic acid fermentation is a quite exergonic process with a slightly negative free Gib...
by victor
Wed Jun 04, 2008 10:20 am
Forum: Microbiology
Topic: Identification test for RHIZOBIUM
Replies: 2
Views: 7113

just inoculate the mixture/suspension of the root node extract into YEMA (yeast extract mannitol agar) medium added with some kinda of indicator dye (if I'm not mistaken is Congo Red). Then after 48-72h there'll be some colonies there. Rhizobium colonies are the colonies that absorb the red pigment ...
by victor
Wed May 21, 2008 2:04 pm
Forum: Molecular Biology
Topic: THE NATURE OF ENZYMES
Replies: 10
Views: 27949

Yup, it does bind the substrate by making a suitable shape for the substrate (proximity & orientation effect). Then it places the nearby active groups in the substrate to the proper amino acid sidechain in the active site (strain & distortion effect). This strain n distortion of the substrat...
by victor
Wed May 21, 2008 11:47 am
Forum: Microbiology
Topic: Soil Science
Replies: 5
Views: 7619

Re: Soil Science

Hi all, i've got many doubts which are not cleared and i sincerely hoped that there will be replies to my questions posted. My questions are as follow: 1. How does the properties of soil (soil microbes, soil chemistry,etc.) affect the rate of decomposition of dead bodies? The bodies are not buried....
by victor
Fri Jan 25, 2008 5:11 am
Forum: Cell Biology
Topic: Repiratory poisons
Replies: 4
Views: 5009

I think amytal is also listed as a respiratory poison because it's an irreversible inhibitor for NADH dehydrogenase (complex I).
Rotenone (if I'm not mistaken) is also works like oligomicine. It blocks the FoF1 ATP synthase, so even though there's a PMF, the FoF1 complex would not synthesize any ATPs.