Search found 2072 matches

by victor
Wed Nov 05, 2008 2:25 pm
Forum: Microbiology
Topic: Heterotrophs and Carbon Fixation
Replies: 7
Views: 10587

Yeah, that thing is Plantanimal :lol:
by victor
Wed Nov 05, 2008 2:09 pm
Forum: Microbiology
Topic: My undergraduate thesis project - Lactose metabolism
Replies: 2
Views: 4451

First of all, I want to do an identification towards this bacteria until the genus, because at least I have to know which phylum that this bacteria belong to. After that I want to do an beta-galactosidase enzyme assay for this bacteria and compare it with the same assay on E.coli strain K12. Any sug...
by victor
Tue Nov 04, 2008 3:10 pm
Forum: Microbiology
Topic: My undergraduate thesis project - Lactose metabolism
Replies: 2
Views: 4451

My undergraduate thesis project - Lactose metabolism

Hi, my name is Victor and I'm about to start my research project for an undergraduate thesis. The research theme is to elucidate biochemical pathway of lactose utilization by bacteria from Sermo dam isolate. The background which made me decide to start a research about it is when I test this strain'...
by victor
Tue Nov 04, 2008 2:11 pm
Forum: Microbiology
Topic: AMPHITRICHOUS MOVEMENT
Replies: 3
Views: 5860

I can think it's probably by the direction of flagellar spin. Bacteria move by 'spinning' their flagella and thus the arrangement of flagellin helices which construct flagella is in certain direction (either right spin or left spin). So, I can say that if the forward movement of bacteria is caused b...
by victor
Tue Nov 04, 2008 1:56 pm
Forum: Microbiology
Topic: Heterotrophs and Carbon Fixation
Replies: 7
Views: 10587

Well I can say it's still called as heterotrophs (chemoorgano heterotrophs), because in order to generate those CO2, they still need organic molecules as the sole electron donor. And I can say that mixotrophs are organisms which do not belong to these 4 nutrition types: - Photolyto autotrophs - Phot...
by victor
Fri Oct 31, 2008 2:26 pm
Forum: Microbiology
Topic: Bacteria in the air?
Replies: 9
Views: 15157

well I can say that it depends on the locations. Like in my country (tropical country), tiny things in the air are so many and I would say they're not bacteria. They're fungal spores, which ready to contaminate every culture medium if the person who works carelessly :p
by victor
Fri Oct 31, 2008 1:35 pm
Forum: Microbiology
Topic: Heterotrophs and Carbon Fixation
Replies: 7
Views: 10587

Yup, it's possible. The example can be seen in industrial fermentation of citric acid by Aspergillus niger . In order to produce citric acid, the CO2 produced from pyruvate decarboxylation is re-used again by combining it with another pyruvate to produce oxaloacetate (by the action of pyruvate carbo...
by victor
Mon Jul 21, 2008 5:08 am
Forum: Molecular Biology
Topic: DMSO in PCR
Replies: 1
Views: 8654

DMSO in PCR

Hi there, I just wondering while I'm doing PCR for beta-thalassemia mutation detections and my friend doing for alpha-thalassemia. I saw that she use DMSO solution in the PCR mix while I'm told it's not necessary to add DMSO in mine. When I ask her, she said that alpha-globin gene contains a relativ...
by victor
Thu Jul 17, 2008 3:33 am
Forum: Site Suggestions
Topic: Immunology?
Replies: 33
Views: 29698

Re:

Dustfinger wrote:Hey Dr.Stein where are you ?


Dr.Stein is a lecturer in Biology Faculty at Gadjah Mada University in 'Yogykarta', Indonesia (I still remember who write this Yogykarta, hahaha...). I can say that she lives in the Animal Physiology Lab and she is my lecturer :mrgreen:
by victor
Thu Jul 17, 2008 3:12 am
Forum: Zoology Discussion
Topic: Cats
Replies: 2
Views: 2757

Hmm..I'm curious for it too..
try to bring your cat close when you slice onions and see whether your cat cry or not.
by victor
Wed Jul 16, 2008 2:04 am
Forum: Molecular Biology
Topic: gel extraction problem
Replies: 4
Views: 3990

hmm..I should note that when I extracting DNA :)
Thanks.
by victor
Wed Jul 16, 2008 2:02 am
Forum: Molecular Biology
Topic: plasma protein precipitation
Replies: 1
Views: 4145

1. Use RBC lysis Buffer about 3x blood volume and incubate for 10 minutes and centrifuge at 1500 rpm; 10 minutes (rt) and after that, pour the supernatant --> do this 3 times. 2. Vortex the pellet and use Cell lysis solution (about 625 uL for 2,5 mL blood) & 2,5 uL RNase --> incubate for 30 minu...