Search found 65 matches

by SororSaudade
Fri Dec 15, 2006 10:56 am
Forum: Molecular Biology
Topic: minimum possible size of a plasmid
Replies: 2
Views: 2225

minimum possible size of a plasmid

Hello!

can someboy tell me what's the minimum size for a DNA fragment to circularize?

Thanks :)
by SororSaudade
Wed Dec 06, 2006 9:17 pm
Forum: Molecular Biology
Topic: Living forever question
Replies: 5
Views: 3029

In fact there are evidences that telomeres (the "ends" of the chromosomes) may be related to the cells survival... they get smaller every new cell division untill a certain point at which the cell dies. Some cencerous cells have large amounts of an enzyme (telomerase) which inhibits this s...
by SororSaudade
Fri Nov 17, 2006 4:07 pm
Forum: Molecular Biology
Topic: pJIT60 nucleotide sequence
Replies: 2
Views: 2372

Thank you VERY much! :D
by SororSaudade
Fri Nov 17, 2006 12:00 pm
Forum: Molecular Biology
Topic: pJIT60 nucleotide sequence
Replies: 2
Views: 2372

pJIT60 nucleotide sequence

Hello again,

does anybody have (or knows where i can find it) the whole sequence of the pJIT 60 plasmid?

Thanks :)
by SororSaudade
Thu Nov 16, 2006 3:56 pm
Forum: Molecular Biology
Topic: Cloning SEKDEL by PCR
Replies: 0
Views: 991

Cloning SEKDEL by PCR

Hi everybody! Has anyone ever tried to clone the sequence SEKDEL (for ER directioning) by PCR? I was wondering if I could get some help with the primer design and some other technical issues (a link or a paper where i can find some decent description of the procedure would also be very very helpful)...